Dr. Amy Ham, assistant professor of pharmacy, was recently informed that her manuscript, “Label-Free Quantitation of Protein Modifications by Pseudo-Selected Reaction Monitoring with Internal Reference Peptides,” was accepted for publication in the American Chemical Society’s Journal of Proteome Research. Dr. Ham and her team describe a method using an ion trap mass spectrometer for label-free quantitation of protein modifications using a targeted MS/MS approach with extraction of transition ions for pseudo-selective reaction monitoring (pSRM) and the use of non-modified peptides within the same protein of interest for normalization. The utility of the method was demonstrated by comparing this approach to the more traditional use of stable-isotope dilution (SID) using the well-studied phosphorylation of epidermal growth factor receptor (EGFR) as an example that could also be verified by commonly used western blot analysis using site-specific antibodies.
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About Belmont UniversityRanked No. 7 in the Regional Universities South category and named for the fifth consecutive year as one of the top “Up-and-Comer” universities by U.S. News & World Report, Belmont University consists of approximately 6,650 students who come from every state and 25 countries. Committed to being a leader among teaching universities, Belmont brings together the best of liberal arts and professional education in a Christian community of learning and service. The university’s purpose is to help students explore their passions and develop their talents to meet the world’s needs, a fact made evident in the University’s hometown, Nashville, where students served more than 60,000 hours of community service (valued at $450,000) during the last academic year. Belmont is also home to the World Cup champion Enactus team, a group of 42 student leaders committed to using the power of entrepreneurial action to transform lives and shape a better, more sustainable world. With more than 80 areas of study, 23 master’s programs and five doctoral degrees, there is no limit to the ways Belmont University can expand an individual's horizon. For more information visit www.belmont.edu